Eschbach Sébastien H, Lafontaine Daniel A
Department of Biology, Faculty of Science, RNA Group, Université de Sherbrooke, Sherbrooke, QC, Canada.
Methods Mol Biol. 2014;1086:255-64. doi: 10.1007/978-1-62703-667-2_15.
The study of biologically relevant native RNA structures is important to understand their cellular function(s). Native gel electrophoresis provides information about such native structures in solution as a function of experimental conditions. The application of native gel electrophoresis in a comparative manner allows to obtain precise information on relative angles subtended between given pair of stems in an RNA molecule. By adapting this approach, it is possible to obtain very specific structural information such as the amplitude of dihedral angles and helical rotation. As an example, we will describe how native gel electrophoresis can be used to study the folding of the S-adenosylmethionine (SAM) sensing riboswitch.
研究具有生物学相关性的天然RNA结构对于理解其细胞功能很重要。天然凝胶电泳可提供有关溶液中此类天然结构与实验条件关系的信息。以比较的方式应用天然凝胶电泳能够获取关于RNA分子中给定茎对之间相对夹角的精确信息。通过采用这种方法，有可能获得非常具体的结构信息，如二面角的幅度和螺旋旋转。例如，我们将描述如何利用天然凝胶电泳来研究S-腺苷甲硫氨酸（SAM）感应核糖开关的折叠。
