Olsvik O, Solberg R, Bergan T
Acta Pathol Microbiol Immunol Scand B. 1985 Aug;93(4):255-62.
Enterotoxigenic Escherichia coli of human and procine origin were characterized with respect to their O and H antigens, fimbrial antigens, and type of enterotoxin produced. Enterotoxin production was determined by bioassay (infant mice) and enzyme-linked immunoassay (ELISA). The presence of genes coding for the enterotoxins was determined by DNA-DNA hybridization. The number and molecular size of plasmids in the enterotoxigenic strains were determined by gel electrophoresis. Strains with the same serological pattern showed different plasmid profiles and could thereby easily be separated. One strain of porcine origin possessed only one plasmid, but produced both heat-labile enterotoxin and the F4 antigen (formerly K88), which is an unusual combination.
对源自人和猪的产肠毒素大肠杆菌进行了O抗原、H抗原、菌毛抗原以及所产肠毒素类型的鉴定。通过生物测定法（幼鼠）和酶联免疫吸附测定法（ELISA）测定肠毒素的产生。通过DNA-DNA杂交确定编码肠毒素的基因的存在。通过凝胶电泳确定产肠毒素菌株中质粒的数量和分子大小。具有相同血清学模式的菌株显示出不同的质粒图谱，因此可以很容易地进行区分。一株源自猪的菌株仅拥有一个质粒，但却产生不耐热肠毒素和F4抗原（以前称为K88），这是一种不寻常的组合。
