Tan K H, Keresztes-Nagy S, Frankfater A
Biochemistry. 1975 Sep 23;14(19):4280-6. doi: 10.1021/bi00690a022.
The pH dependency of the dissociation of oxyhemerythrin has been studied by frontal gel chromatography on Sephadex G-75. The extent of dissociation is markedly pH dependent increasing below pH 6.0 and above pH 8.8. In addition, the nature of the dissociation reaction undergoes dramatic change with pH. Below pH 6.4 the rate of equilibration between species is slow relative to their time of passage through the column and they are thus resolved on chromatography. Above pH 6.6 the rate of equilibration is rapid and the various forms of hemerythrin are not resolved on migration through the column. Below pH 7.4 the dissociation is an all-or-none process with no detectable intermediates. Above pH 8.0 several intermediate species can be detected. Values for Keq and deltaG degrees are presented for various forms of oxyhemerythrin at the several pH's studied.
通过在葡聚糖凝胶G - 75上进行前沿凝胶色谱法研究了氧合蚯蚓血红蛋白解离的pH依赖性。解离程度明显依赖于pH，在pH 6.0以下和pH 8.8以上时增加。此外，解离反应的性质随pH发生显著变化。在pH 6.4以下，物种之间的平衡速率相对于它们通过柱子的时间较慢，因此它们在色谱上得以分离。在pH 6.6以上，平衡速率很快，蚯蚓血红蛋白的各种形式在通过柱子迁移时无法分离。在pH 7.4以下，解离是一个全或无的过程，没有可检测到的中间体。在pH 8.0以上，可以检测到几种中间物种。给出了在所研究的几个pH值下各种形式的氧合蚯蚓血红蛋白的Keq和ΔG°值。
