Endo T, Imai K, Yachi A, Nakajima K, Watanabe T
Cancer. 1985 Nov 1;56(9):2205-11. doi: 10.1002/1097-0142(19851101)56:9<2205::aid-cncr2820560912>3.0.co;2-i.
The serum from a colonic cancer patient was examined showing anti-CEA (carcinoembryonic antigen) activity but not anti-NCA (nonspecific cross-reacting antigen) activity. Conventional immunoelectrophoresis and radioimmunoelectrophoresis in conjunction with iodine 125-labeled CEA strongly suggested that this serum contained M-protein (IgG kappa). Most of the anti-CEA activity was found in the M-component using radioimmunoelectrophoresis, suggesting the monoclonal nature of anti-CEA autoantibodies. With the use of this serum the histologic distribution of the antigen by the immunoperoxidase method was demonstrated for the first time. Anti-CEA specificity of this serum was confirmed by the fact that the absorbed antibody with purified CEA gave negative staining of the serial sections of the tissues that formerly had immunostaining. Interestingly, this distribution was almost superimposed on that detected by the murine monoclonal anti-CEA antibody AS802. The data suggested that the antibody in the serum reacted with the CEA molecule in the tissue sections, the majority of which were found in the cancerous tissues.
对一名结肠癌患者的血清进行检测，结果显示其具有抗癌胚抗原（CEA）活性，但不具有抗非特异性交叉反应抗原（NCA）活性。常规免疫电泳和放射免疫电泳结合碘125标记的CEA强烈提示该血清含有M蛋白（IgG κ）。利用放射免疫电泳发现大部分抗CEA活性存在于M成分中，这表明抗CEA自身抗体具有单克隆性质。使用该血清首次通过免疫过氧化物酶法证实了抗原的组织学分布。该血清的抗CEA特异性通过以下事实得到证实：用纯化的CEA吸收抗体后，先前有免疫染色的组织连续切片呈现阴性染色。有趣的是，这种分布几乎与鼠单克隆抗CEA抗体AS802检测到的分布重叠。数据表明血清中的抗体与组织切片中的CEA分子发生反应，其中大部分存在于癌组织中。
