Laboratory for Comparative Genomics and Bioinformatics, College of Life Science, Nanjing Normal University, Nanjing 210046, PR China; Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, PR China.
The Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Institute of Life Science, Southeast University, Nanjing 210009, PR China.
Genomics. 2014 Jan;103(1):147-53. doi: 10.1016/j.ygeno.2013.10.002. Epub 2013 Oct 16.
The transforming growth factor β induced gene (TGFBIG) encodes a protein (TGFBI) which plays important roles in many biological processes. However, no TGFBIG homolog has been reported in B. belcheri. Here, we identified a TGFBI-like gene from B. belcheri and extensively studied the evolutionary history of TGFBI family. We found that the amphioxus genome contains a TGFBIG homolog designated as AmphiTGFBI which encodes a protein with 5 Fas1 domains. The TGFBIGs were present in a common ancestor with Amphimedon queenslandica. We also demonstrated expression patterns of AmphiTGFBI in five amphioxus tissues. Interestingly, the gene structures and conserved motifs of invertebrate TGFBIGs were found to present regular changes in the evolution. Positive selection and Fas1 domain loss might cause the regular changes of gene structures and conserved motifs in invertebrate TGFBIGs during evolution. Together, our findings provided an insight into the evolution of the TGFBI family.
转化生长因子 β 诱导基因（TGFBIG）编码一种蛋白（TGFBI），在许多生物学过程中发挥重要作用。然而，尚未有报道称文昌鱼中存在 TGFBIG 同源物。在这里，我们从文昌鱼中鉴定出一个 TGFBI 样基因，并对 TGFBI 家族的进化历史进行了广泛研究。我们发现，文昌鱼基因组中存在一个 TGFBIG 同源物，命名为 AmphiTGFBI，它编码一个具有 5 个 Fas1 结构域的蛋白。TGFBIG 存在于与昆士兰文昌鱼的共同祖先中。我们还在文昌鱼的五个组织中展示了 AmphiTGFBI 的表达模式。有趣的是，我们发现无脊椎动物 TGFBIG 的基因结构和保守基序在进化过程中呈现出有规律的变化。正选择和 Fas1 结构域丢失可能导致无脊椎动物 TGFBIG 在进化过程中基因结构和保守基序的有规律变化。总之，我们的研究结果为 TGFBI 家族的进化提供了新的见解。
