Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA; Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA.
J Microbiol Methods. 2013 Dec;95(3):366-7. doi: 10.1016/j.mimet.2013.10.005. Epub 2013 Oct 16.
The ferrozine assay is a widely used colorimetric method for determining soluble iron concentrations. We provide evidence for a heretofore unrecognized interference of ferric ions (Fe(3+)) on ferrous iron (Fe(2+)) measurements performed in the dark. Fe(3+) concentrations affected the absorbance measurements, which linearly increased with incubation time.
铁嗪测定法是一种广泛用于测定可溶性铁浓度的比色法。我们提供了以前未被认识到的铁离子（Fe(3+)）对黑暗中进行的亚铁离子（Fe(2+)）测量的干扰的证据。Fe(3+)浓度影响吸光度测量，其随孵育时间呈线性增加。
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