Department of Biological Sciences, Dartmouth College, Hanover, NH, USA.
Methods Mol Biol. 2023;2665:31-36. doi: 10.1007/978-1-0716-3183-6_3.
A sensitive FerroZine assay is used to measure the membrane-bound ferric-chelate reductase activity in the Arabidopsis thaliana roots. In Arabidopsis, FRO2 (FERRIC CHELATE REDUCTASE 2) encodes the Fe(III) chelate reductase and its expression is induced by iron deficiency. As FRO2 reduces Fe(III) to soluble Fe(II), the resulting Fe(II) forms a purple-colored complex with the dye FerroZine. The concentration of the Fe(II)-FerroZine is directly proportional to the absorbance at 562 nm.
采用灵敏的 FerroZine 法测定拟南芥根中膜结合的三价铁螯合还原酶活性。在拟南芥中，FRO2（FERRIC CHELATE REDUCTASE 2）编码三价铁螯合还原酶，其表达受缺铁诱导。由于 FRO2 将 Fe(III)还原为可溶性 Fe(II)，生成的 Fe(II)与染料 FerroZine 形成紫色络合物。Fe(II)-FerroZine 的浓度与 562nm 处的吸光度成正比。
