Fernandez Cristina E, Obi-onuoha Izundu C, Wallace Charles S, Satterwhite Lisa L, Truskey George A, Reichert William M
Department of Biomedical Engineering, Duke University, 136 Hudson Hall, 101 Science Drive, Durham, NC 27708, USA.
Department of Biomedical Engineering, Duke University, 136 Hudson Hall, 101 Science Drive, Durham, NC 27708, USA.
Acta Biomater. 2014 Feb;10(2):893-900. doi: 10.1016/j.actbio.2013.10.004. Epub 2013 Oct 16.
Patients with coronary artery disease (CAD) are the primary candidates to receive small-diameter tissue-engineered blood vessels (TEBVs). Peripheral blood derived endothelial progenitor cells (EPCs) from CAD patients (CAD EPCs) represent a minimally invasive source of autologous cells for TEBV endothelialization. We have previously shown that human CAD EPCs are highly proliferative and express many of the hallmarks of mature and healthy endothelial cells; however, their behavior on stromal cells that comprise the media of TEBVs has not yet been evaluated. Primary CAD EPCs or control human aortic endothelial cells (HAECs) were seeded over confluent, quiescent layers of human smooth muscle cells (SMCs) using a direct co-culture model. The percent coverage, adhesion strength, alignment under flow and generation of flow-induced nitric oxide of the seeded CAD EPCs were compared to that of HAECs. The integrin-binding profile of CAD EPCs was also evaluated over a layer of confluent, quiescent SMCs. Direct comparison of our CAD EPC results to analogous co-culture studies with cord blood EPCs show that both types of blood-derived EPCs are viable options for endothelialization of TEBVs.
冠状动脉疾病（CAD）患者是接受小口径组织工程血管（TEBVs）的主要候选者。来自CAD患者的外周血源性内皮祖细胞（EPCs）（CAD EPCs）是用于TEBV内皮化的自体细胞的微创来源。我们之前已经表明，人类CAD EPCs具有高度增殖性，并表达许多成熟和健康内皮细胞的特征；然而，它们在构成TEBVs中膜的基质细胞上的行为尚未得到评估。使用直接共培养模型，将原代CAD EPCs或对照人主动脉内皮细胞（HAECs）接种在汇合、静止的人平滑肌细胞（SMCs）层上。将接种的CAD EPCs的覆盖百分比、黏附强度、流动状态下的排列以及流动诱导的一氧化氮生成与HAECs进行比较。还在汇合、静止的SMCs层上评估了CAD EPCs的整合素结合谱。将我们的CAD EPC结果与脐带血EPCs的类似共培养研究进行直接比较，结果表明这两种血液源性EPCs都是TEBVs内皮化的可行选择。
