采用漱口液采集的口腔漱口标本中分泌型白细胞蛋白酶抑制剂（SLPI）的定量分析。
Quantification of secretory leukocyte protease inhibitor (SLPI) in oral gargle specimens collected using mouthwash.
机构信息
Department of Cancer Epidemiology, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA; Center for Infection Research in Cancer, Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612, USA.
出版信息
J Immunol Methods. 2013 Dec 31;400-401:117-21. doi: 10.1016/j.jim.2013.10.005. Epub 2013 Oct 17.
BACKGROUND
Secretory leukocyte protease inhibitor (SLPI) is an innate immunity-associated protein known to inhibit HIV transmission, and is thought to inhibit a variety of infectious agents, including human papillomaviruses (HPVs). We aimed to optimize an established ELISA-based SLPI quantification assay for use with oral gargle specimens collected using mouthwash, and to assess preliminary associations with age, smoking status, and alcohol intake.
METHODS
Oral gargle supernatants from 50 individuals were used to optimize the Human SLPI Quantikine ELISA Kit. Sample suitability was assessed and quality control analyses were conducted.
RESULTS
Salivary SLPI was successfully recovered from oral gargles with low intra-assay and high inter-individual variability. Initial measurements showed that salivary SLPI varied considerably across individuals, and that SLPI was inversely associated with age.
CONCLUSIONS
This optimized assay can be used to examine the role of SLPI in the acquisition of oral HPV and other infections.
背景
分泌型白细胞蛋白酶抑制剂（SLPI）是一种与先天免疫相关的蛋白，已知其能够抑制 HIV 的传播，并且被认为能够抑制多种感染因子，包括人乳头瘤病毒（HPV）。我们旨在优化一种已建立的基于 ELISA 的 SLPI 定量检测方法，以用于使用漱口水采集的口腔漱口液标本，并初步评估其与年龄、吸烟状况和饮酒量的相关性。
方法
使用 50 名个体的口腔漱口液上清液来优化人 SLPI Quantikine ELISA 试剂盒。评估了样品的适用性并进行了质量控制分析。
结果
成功从口腔漱口液中回收了唾液 SLPI，具有较低的内检测变异性和较高的个体间变异性。初步测量结果表明，唾液 SLPI 在个体间差异很大，并且 SLPI 与年龄呈负相关。
结论
这种优化的检测方法可用于研究 SLPI 在口腔 HPV 和其他感染中的作用。
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