Ryazanov A G
FEBS Lett. 1985 Nov 11;192(1):131-4. doi: 10.1016/0014-5793(85)80058-2.
One of the 3 major RNA-binding proteins of rabbit reticulocytes, a polypeptide of 36 kDa, is identified as glyceraldehyde-3-phosphate dehydrogenase (GAPD). This fact was deduced from the identity of molecular masses, one-dimensional peptide maps and isoelectric points of the 36 kDa protein and GAPD from rabbit muscle. It is concluded that GAPD can bind rather unspecifically different RNAs and polynucleotides. This means that GAPD, like other RNA-binding proteins, can form loose dynamic complexes with polyribosomes. Association of such a kind may be used for compartmentation of glycolysis near polyribosomes.
兔网织红细胞的三种主要RNA结合蛋白之一是一种36 kDa的多肽，被鉴定为甘油醛-3-磷酸脱氢酶（GAPD）。这一事实是根据兔肌肉中36 kDa蛋白与GAPD的分子量、一维肽图和等电点的一致性推断出来的。得出的结论是，GAPD可以相当非特异性地结合不同的RNA和多核苷酸。这意味着，GAPD与其他RNA结合蛋白一样，可以与多核糖体形成松散的动态复合物。这种结合可能用于在多核糖体附近对糖酵解进行区室化。
