[Genetic restriction specificity of suppressor T cell circuit--analyses by allogeneic bone marrow chimeras].

To study adaptive differentiation phenomenon of T lymphocytes, a number of irradiation chimeras constructed from various combinations of marrow cells and recipient mice were prepared, and Ly-1 T cell suppressor inducer factor (Ly-1 TsiF) and Ly-2 T cell suppressor factor (Ly-2 TsF) from these chimeras were analyzed for their ability to inhibit in vitro primary antibody responses to sheep erythrocytes (SRBC) of spleen cells from various strains of mice. AKR mice reconstructed with B10 marrow cells, [B10----AKR] chimeras, were able to produce Ly-1 TsiF and Ly-2 TsF following hyper-immunization with SRBC. The Ly-1 TsiF from [B10----AKR] chimeras suppressed antigen-specifically primary antibody responses to SRBC generated in the spleen cells of the same Igh-Vb haplotype as that of donor strains but not those in the cells of different Igh-V type. The Ly-2 TsF from [B10----AKR] chimeras was also shown to be donor restricted, but in this case restriction molecules were demonstrated to be H-2 products. Furthermore, the Ly-2 TsF from [B10----AKR] chimeras suppressed the responses seen in B10.A (3R) and B10.A (5R) mice but not those in B10.A (4R) mice, indicating that identity between the factor producing cells and target cells was required at the left side from E beta locus of the H-2 for the factor to show suppressive activity and that putative I-Jb was not involved in the suppression. Very similar results were obtained when Ly-1 TsiF and Ly-2 TsF from [B6----BALB/c] and [BALB/c----B6] chimeras were analyzed.(ABSTRACT TRUNCATED AT 250 WORDS)