Mikulášová Mária, Valáriková Jana, Dušinský Roman, Chovanová Romana, Belicová Anna
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15, Bratislava, Slovakia.
Folia Microbiol (Praha). 2014 May;59(3):223-7. doi: 10.1007/s12223-013-0286-y. Epub 2013 Oct 19.
Staphylococcus xylosus, Staphylococcus equorum, and Staphylococcus epidermidis strains were isolated from Bryndza cheese and identified using PCR method. The antimicrobial susceptibility of these strains was assessed using disc diffusion method and broth microdilution method. The highest percentage of resistance was detected for ampicillin and oxacillin, and in contrary, isolates were susceptible or intermediate resistant to ciprofloxacin and chloramphenicol. Fourteen of the S. xylosus isolates (45%) and eleven of the S. equorum isolates (41%) exhibited multidrug resistance. None of the S. epidermidis isolate was multiresistant. The phenotypic resistance to oxacillin was verified by PCR amplification of the gene mecA.
从布伦扎奶酪中分离出木糖葡萄球菌、马胃葡萄球菌和表皮葡萄球菌菌株,并采用聚合酶链反应(PCR)方法进行鉴定。使用纸片扩散法和肉汤微量稀释法评估这些菌株的抗菌药敏性。氨苄西林和苯唑西林的耐药率最高,相反,分离株对环丙沙星和氯霉素敏感或呈中度耐药。14株木糖葡萄球菌分离株(45%)和11株马胃葡萄球菌分离株(41%)表现出多重耐药性。表皮葡萄球菌分离株均无多重耐药。通过对mecA基因进行PCR扩增验证了对苯唑西林的表型耐药性。