Okanoue T, Ohta M, Ou O, Kachi K, Kagawa K, Yuki T, Okuno T, Takino T, French S W
Lab Invest. 1985 Nov;53(5):534-40.
Livers from 12 mice fed griseofulvin for 4 to 6 months were perfused in situ with a detergent solution to extract lipid membranes leaving the cytoskeleton intact. Seven control mice were similarly studied. After 30 to 120 minutes perfusion, liver samples were examined by scanning electron microscopy and transmission electron microscopy. By light microscopy, Mallory bodies (MBs) were observed in pericentral hepatocytes. These were confirmed by transmission electron microscopy. Intermediate filaments (IFs) were observed in close apposition to MBs. Numerous IFs were seen throughout the cytoplasm. The 3-dimensional organization of the cytoskeleton and MBs were clearly visualized by scanning electron microscopy. The IFs were disorganized in the hepatocytes and formed small MBs in griseofulvin-treated mice. In the case of hepatocytes containing large MBs, there was an apparent decrease in the concentration of IFs. Transition forms of dense networks of IFs between the normal cytoskeleton and the MBs were noted in the cytoplasm between small MBs and the nucleus and also at the cell border. The IFs connected to the nucleus and invaded the MBs. The MBs appeared to form as a result of condensation or collapse of the IFs.
对12只喂食灰黄霉素4至6个月的小鼠的肝脏进行原位灌注，用去污剂溶液提取脂质膜，使细胞骨架保持完整。对7只对照小鼠进行了类似研究。灌注30至120分钟后，通过扫描电子显微镜和透射电子显微镜检查肝脏样本。通过光学显微镜观察到中央周围肝细胞中有马洛里小体（MBs）。透射电子显微镜证实了这些小体。观察到中间丝（IFs）与MBs紧密相邻。在整个细胞质中都可见到大量的中间丝。通过扫描电子显微镜可以清晰地看到细胞骨架和MBs的三维结构。在灰黄霉素处理的小鼠中，肝细胞中的中间丝紊乱，并形成了小的MBs。在含有大MBs的肝细胞中，中间丝的浓度明显降低。在小MBs与细胞核之间的细胞质以及细胞边界处，可观察到正常细胞骨架和MBs之间中间丝密集网络的过渡形式。中间丝与细胞核相连并侵入MBs。MBs似乎是由中间丝的凝聚或塌陷形成的。
