Ma Xiuqin, Chen Ruhua, Liu Xiufang, Xie Jin, Si Keyun, Duan Lirong
Department of Respiratory Disease, Yixing People Hospital, Yixing 214200, China.
Afr J Tradit Complement Altern Med. 2013 Apr 12;10(3):442-8. doi: 10.4314/ajtcam.v10i3.10. eCollection 2013.
The current study aims to investigate the effects of matrine on the JAK-STAT signaling transduction pathways in bleomycin (BLM)-induced pulmonary fibrosis (PF) and to explore its action mechanism. A total of 72 male C57BL/6 mice were randomized into the control, model, and treatment groups. PF models were established by instilling BLM intratracheally. The treatment group was given daily matrine through gastric lavage. Six mice were sacrificed in each group at 3, 7, 14, and 28 days. The lung tissues were observed using hematoxylin-eosin staining. The expression of JAK, STAT1, and STAT3 was observed using immunohistochemistry and then determined using real-time polymerase chain reaction. Alveolitis and PF significantly improved in the treatment group compared with the model group (P < 0.05). The expression of JAK, STAT1, and STAT3 in the model group increased at day 7, peaked at day 14 and then decreased, but the expression was still higher than that in the control group at day 28 (P < 0.05). The three indices in the treatment group were significantly lower than those in the model group at any detection time point (P < 0.05). PF causes high expression of JAK, STAT1, and STAT3. Matrine exerts an anti-PF effect by inhibiting the JAK-STAT signaling transduction pathways.
本研究旨在探讨苦参碱对博来霉素（BLM）诱导的肺纤维化（PF）中JAK-STAT信号转导通路的影响，并探讨其作用机制。将72只雄性C57BL/6小鼠随机分为对照组、模型组和治疗组。通过气管内注入BLM建立PF模型。治疗组通过灌胃给予苦参碱。每组在第3、7、14和28天处死6只小鼠。用苏木精-伊红染色观察肺组织。用免疫组织化学观察JAK、STAT1和STAT3的表达，然后用实时聚合酶链反应进行测定。与模型组相比，治疗组的肺泡炎和PF明显改善（P<0.05）。模型组JAK、STAT1和STAT3的表达在第7天增加，在第14天达到峰值，然后下降，但在第28天仍高于对照组（P<0.05）。治疗组在任何检测时间点的这三个指标均明显低于模型组（P<0.05）。PF导致JAK、STAT1和STAT3高表达。苦参碱通过抑制JAK-STAT信号转导通路发挥抗PF作用。
