菟丝子中鞣质的提取工艺及其抗乳头瘤活性研究。
Study on extraction process of tannins from Semen Cuscutae and their anti-papilloma activity.
作者信息
Wang Jiangning, Li Xiaorong, Gao Lei
机构信息
Central Laboratory, Luhe Teaching Hospital of the Capital Medical University, Beijing, 101100, China.
出版信息
Afr J Tradit Complement Altern Med. 2013 Apr 12;10(3):469-74. doi: 10.4314/ajtcam.v10i3.13. eCollection 2013.
The objective of this paper was to study the extraction methods of tannin constituents from Semen Cuscutae and their anti-papilloma effects. Single factor test and orthogonal design methods were used to determine the optimal extraction method; the mouse skin papilloma model induced by DMBA/croton oil was established, which was a classic two-stage carcinogenesis model being used to observe and evaluate the anti-carcinogenic effects of tannins extracted from Semen Cuscutae in different stages. The optimal extraction method of Semen Cuscutae was a 20-fold volume of solvent, a temperature of 50 °C, three times of extraction, with 20 min each, skin papilloma experiment revealed that the number of bearing tumors gradually reduced, and the inhibition rate gradually increased with the increase of dose, in the high-dose group, its inhibition rate reached 70.2%. Tannin extract from Semen Cuscutae has an obvious inhibitory effect on skin papilloma development.
本文的目的是研究菟丝子中鞣质成分的提取方法及其抗乳头瘤作用。采用单因素试验和正交设计方法确定最佳提取方法；建立了由二甲基苯并蒽/巴豆油诱导的小鼠皮肤乳头瘤模型，这是一种经典的两阶段致癌模型，用于观察和评价不同阶段从菟丝子中提取的鞣质的抗癌作用。菟丝子的最佳提取方法为溶剂体积为20倍，温度为50℃，提取3次，每次20分钟，皮肤乳头瘤实验表明，荷瘤数量随剂量增加逐渐减少，抑制率逐渐升高，高剂量组抑制率达70.2%。菟丝子鞣质提取物对皮肤乳头瘤的发展有明显的抑制作用。
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