Lewis L N, Mezei C
Muscle Nerve. 1985 Feb;8(2):120-4. doi: 10.1002/mus.880080207.
To investigate whether various myelin markers could detect pathological changes in myelination, the activity of 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and the level of PO protein were compared in the peripheral nervous system (PNS) of the dystrophic mouse and chicken and their phenotypically normal siblings. The same comparison was made for subcellular fractions of these nerves. The level of PO protein and the activity of CNP were normal in the spinal roots of sciatic nerves of the 129B6F1/Jdy/dy strain of dystrophic mouse. These parameters were slightly but significantly lower in the spinal roots of the 129REJ/dy/dy strain of dystrophic mouse. These results suggest that the 129B6F1/Jdy/dy mutant and the 129REJ/dy/dy mutant mouse might not have the same type of biochemical abnormality in their PNS. The specific activity of CNP and the proportion of PO protein increased significantly in the various regions of the PNS of 1-day-old chicks when compared to that of the 18-day-old embryos. Subcellular fractionation of peripheral nerves indicated that these components were enriched in the crude myelin fraction. These results support the conclusion that PO protein and CNP are suitable markers of myelinogenesis in the PNS of the chick. These parameters were normal in the line 413 dystrophic chicken. These results do not support the conclusion of a systemic membrane defect in the PNS of the dystrophic mouse and chicken.
为了研究各种髓鞘标记物能否检测髓鞘形成中的病理变化，比较了营养不良小鼠和鸡及其表型正常的同胞的外周神经系统（PNS）中2'-3'-环核苷酸3'-磷酸二酯酶（CNP）的活性和PO蛋白的水平。对这些神经的亚细胞组分进行了同样的比较。129B6F1/Jdy/dy品系营养不良小鼠坐骨神经的脊髓神经根中PO蛋白水平和CNP活性正常。在129REJ/dy/dy品系营养不良小鼠的脊髓神经根中，这些参数略低但有显著差异。这些结果表明，129B6F1/Jdy/dy突变体和129REJ/dy/dy突变体小鼠在其PNS中可能没有相同类型的生化异常。与18日龄胚胎相比，1日龄雏鸡PNS各区域的CNP比活性和PO蛋白比例显著增加。外周神经的亚细胞分级分离表明，这些成分在粗髓鞘组分中富集。这些结果支持PO蛋白和CNP是雏鸡PNS中髓鞘形成的合适标记物这一结论。413品系营养不良鸡的这些参数正常。这些结果不支持营养不良小鼠和鸡的PNS存在系统性膜缺陷这一结论。
