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从兔视网膜分离出的米勒细胞的钠钾激活三磷酸腺苷酶表现出与脑星形胶质细胞相似的钾离子依赖性。

Na+,K+-activated adenosine triphosphatase of isolated Müller cells from the rabbit retina shows a K+ dependence similar to that of brain astrocytes.

作者信息

Reichenbach A, Dettmer D, Reichelt W, Eberhardt W

出版信息

Neurosci Lett. 1985 Sep 6;59(3):281-4. doi: 10.1016/0304-3940(85)90145-4.

DOI:10.1016/0304-3940(85)90145-4
PMID:2414692
Abstract

Müller (glial) cells from the rabbit retina were isolated by means of papain and mechanical dissociation. Their Na+,K+-adenosine triphosphatase (ATPase) activity was measured using a radiochemical method, and its K+ dependence was determined. In contrast to that of photoreceptors (data from the literature), the Na+,K+-ATPase of Müller cells could be shown to increase its activity greatly when the [K+] was enhanced up to 10 mM. The functional implications of this behaviour for the K+ clearance in the retina are discussed.

摘要

通过木瓜蛋白酶和机械解离的方法分离出兔视网膜的 Müller(神经胶质)细胞。使用放射化学方法测量其 Na⁺、K⁺ - 三磷酸腺苷酶(ATP 酶)活性,并确定其对 K⁺ 的依赖性。与光感受器的情况(文献数据)相反,当 [K⁺] 增加到 10 mM 时,Müller 细胞的 Na⁺、K⁺ - ATP 酶活性会显著增加。讨论了这种行为对视网膜中 K⁺ 清除的功能意义。

相似文献

1
Na+,K+-activated adenosine triphosphatase of isolated Müller cells from the rabbit retina shows a K+ dependence similar to that of brain astrocytes.从兔视网膜分离出的米勒细胞的钠钾激活三磷酸腺苷酶表现出与脑星形胶质细胞相似的钾离子依赖性。
Neurosci Lett. 1985 Sep 6;59(3):281-4. doi: 10.1016/0304-3940(85)90145-4.
2
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Neurosci Lett. 1985 Mar 22;55(1):29-34. doi: 10.1016/0304-3940(85)90307-6.
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High Na+ affinity of the Na+,K+ pump in isolated rabbit retinal Müller (glial) cells.
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引用本文的文献

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Intracellular recordings from isolated rabbit retinal Müller (glial) cells.来自分离的兔视网膜穆勒(神经胶质)细胞的细胞内记录。
Pflugers Arch. 1986 Sep;407(3):348-53. doi: 10.1007/BF00585313.
2
Quantitative-morphometric aspects of Bergmann glial (Golgi epithelial) cell development in rats. A Golgi study.大鼠伯格曼胶质细胞(高尔基上皮细胞)发育的定量形态学研究。一项高尔基染色研究。
Anat Embryol (Berl). 1987;177(2):183-8. doi: 10.1007/BF00572543.