Reichenbach A, Dettmer D, Reichelt W, Eberhardt W
Neurosci Lett. 1985 Sep 6;59(3):281-4. doi: 10.1016/0304-3940(85)90145-4.
Müller (glial) cells from the rabbit retina were isolated by means of papain and mechanical dissociation. Their Na+,K+-adenosine triphosphatase (ATPase) activity was measured using a radiochemical method, and its K+ dependence was determined. In contrast to that of photoreceptors (data from the literature), the Na+,K+-ATPase of Müller cells could be shown to increase its activity greatly when the [K+] was enhanced up to 10 mM. The functional implications of this behaviour for the K+ clearance in the retina are discussed.
通过木瓜蛋白酶和机械解离的方法分离出兔视网膜的 Müller(神经胶质)细胞。使用放射化学方法测量其 Na⁺、K⁺ - 三磷酸腺苷酶(ATP 酶)活性,并确定其对 K⁺ 的依赖性。与光感受器的情况(文献数据)相反,当 [K⁺] 增加到 10 mM 时,Müller 细胞的 Na⁺、K⁺ - ATP 酶活性会显著增加。讨论了这种行为对视网膜中 K⁺ 清除的功能意义。