Hassani Seyedeh-Nafiseh, Totonchi Mehdi, Gourabi Hamid, Schöler Hans R, Baharvand Hossein
1 Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology , ACECR, Tehran, Iran .
Stem Cells Dev. 2014 Feb 1;23(3):193-208. doi: 10.1089/scd.2013.0368. Epub 2013 Nov 27.
Human and mouse embryonic stem cells (ESCs) exhibit fundamental differences depicting two distinct states of pluripotency: naive and primed. Mouse ESCs (mESCs) are dependent on leukemia inhibitory factor for growth in culture and possess two active X chromosomes in their female cell lines and correspond to the naive state of pluripotency. Human ESCs (hESCs), however, closely resemble mouse epiblast stem cells and correspond to the primed state. Primed stem cells are dependent on basic FGF for growth and show differentiation bias into different cell lineages. Recent studies have revealed that these two pluripotent states can be interconverted by modifying the culture conditions, although unequivocal evidence for obtaining truly naive hESCs has not been found. Accurate identification of the functions of major pluripotency-related signaling pathways and their cross-talk networks should aid in the successful induction of stable naive pluripotency in human cells.
人类和小鼠胚胎干细胞（ESCs）表现出根本差异，描绘了两种不同的多能性状态：原始态和始发态。小鼠胚胎干细胞（mESCs）在培养中生长依赖白血病抑制因子，在其雌性细胞系中拥有两条活跃的X染色体，对应于原始多能性状态。然而，人类胚胎干细胞（hESCs）与小鼠上胚层干细胞非常相似，对应于始发态。始发态干细胞生长依赖碱性成纤维细胞生长因子（basic FGF），并表现出向不同细胞谱系分化的倾向。最近的研究表明，通过改变培养条件，这两种多能性状态可以相互转换，尽管尚未找到获得真正原始态hESCs的确切证据。准确识别主要多能性相关信号通路的功能及其相互作用网络，应有助于在人类细胞中成功诱导出稳定的原始多能性。
