转运蛋白如何偶联溶质的转运？
How do transporters couple solute movements?
作者信息
Rudnick Gary
机构信息
Department of Pharmacology, Yale University School of Medicine , New Haven, CT , USA.
出版信息
Mol Membr Biol. 2013 Nov;30(7):355-9. doi: 10.3109/09687688.2013.842658.
Abstract
Abstract The availability of high-resolution atomic structures for transport proteins provides unprecedented opportunities for understanding their mechanism of action. The details of conformational change can be deduced from these structures, especially when multiple conformations are available. However, the singular ability of transporters to couple the movement of one solute to that of another requires even more information than what is supplied by a crystal structure. This short commentary discusses how recent biochemical and biophysical studies are beginning to reveal how solute coupling is achieved.
摘要
摘要 转运蛋白高分辨率原子结构的可得性为理解其作用机制提供了前所未有的机会。构象变化的细节可从这些结构中推导出来，尤其是当有多个构象时。然而，转运蛋白将一种溶质的移动与另一种溶质的移动相偶联的独特能力需要比晶体结构所提供的更多信息。本简短评论讨论了最近的生化和生物物理研究如何开始揭示溶质偶联是如何实现的。
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本文引用的文献
1
Transport dynamics in a glutamate transporter homologue.
Nature. 2013 Oct 3;502(7469):114-8. doi: 10.1038/nature12265. Epub 2013 Jun 23.
2
X-ray structures of LeuT in substrate-free outward-open and apo inward-open states.
Nature. 2012 Jan 9;481(7382):469-74. doi: 10.1038/nature10737.
3
Substrate-modulated gating dynamics in a Na+-coupled neurotransmitter transporter homologue.
Nature. 2011 Jun 2;474(7349):109-13. doi: 10.1038/nature09971. Epub 2011 Apr 24.
4
Opposite movement of the external gate of a glutamate transporter homolog upon binding cotransported sodium compared with substrate.
J Neurosci. 2011 Apr 20;31(16):6255-62. doi: 10.1523/JNEUROSCI.6096-10.2011.
5
Structural perspectives on secondary active transporters.
Trends Pharmacol Sci. 2010 Sep;31(9):418-26. doi: 10.1016/j.tips.2010.06.004. Epub 2010 Jul 23.
6
Ion/substrate-dependent conformational dynamics of a bacterial homolog of neurotransmitter:sodium symporters.
Nat Struct Mol Biol. 2010 Jul;17(7):822-9. doi: 10.1038/nsmb.1854. Epub 2010 Jun 20.
7
Inward-facing conformation of glutamate transporters as revealed by their inverted-topology structural repeats.
Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20752-7. doi: 10.1073/pnas.0908570106. Epub 2009 Nov 19.
8
Transport mechanism of a bacterial homologue of glutamate transporters.
Nature. 2009 Dec 17;462(7275):880-5. doi: 10.1038/nature08616. Epub 2009 Nov 18.
9
Antidepressant specificity of serotonin transporter suggested by three LeuT-SSRI structures.
Nat Struct Mol Biol. 2009 Jun;16(6):652-7. doi: 10.1038/nsmb.1602. Epub 2009 May 10.
10
A competitive inhibitor traps LeuT in an open-to-out conformation.
Science. 2008 Dec 12;322(5908):1655-61. doi: 10.1126/science.1166777.
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