Laboratoire de Photochimie et d'Analyse, Faculté des Sciences et Techniques, Université C.A. Diop, Dakar, Sénégal.
Talanta. 2013 Nov 15;116:569-74. doi: 10.1016/j.talanta.2013.07.014. Epub 2013 Jul 11.
A direct Laser Photo-Induced Fluorescence (DL-PIF) method is developed for the determination of two phenylurea pesticides, namely fenuron and diflubenzuron. The DL-PIF method uses a tunable Nd:YAG-OPO Laser to obtain the photoproduct(s) and to simultaneously analyse their fluorescence in a short acquisition time on an intensified CCD camera. Compared to classical PIF methods, the use of a tunable laser improves the selectivity (by choosing the suitable excitation wavelength), increases the sensitivity (due to the high energy of the beam) and also reduces the time of analysis. The analytical performances of this method for the determination of both pesticides are satisfactory in comparison to other classical PIF methods published for the determination of phenylurea pesticides. The calibration curves were linear over one order of magnitude and the limits of detection were in the ng mL(-1) range. Satisfactory recoveries were obtained in the analysis of both pesticides in river and sea water spiked samples.
建立了一种直接激光光致荧光（DL-PIF）方法，用于测定两种苯脲类农药，即灭草隆和除虫脲。DL-PIF 方法使用可调谐 Nd:YAG-OPO 激光器获得光产物，并在短的采集时间内在增强型 CCD 相机上同时分析它们的荧光。与经典的 PIF 方法相比，可调谐激光的使用提高了选择性（通过选择合适的激发波长），增加了灵敏度（由于光束的高能量），并且还缩短了分析时间。与其他用于测定苯脲类农药的经典 PIF 方法相比，该方法在测定这两种农药时具有令人满意的分析性能。校准曲线在一个数量级范围内呈线性，检出限在 ng mL(-1)范围内。在对河水和海水加标样品进行分析时，得到了令人满意的回收率。
