Magakian Iu A, Avetisian A S, Karalova E M
Tsitologiia. 1985 Sep;27(9):1078-85.
Dependence of the staining and absorption spectra of DNA-fuchsin on the dye quality and modes of preparation of the Schiff-reagent was studied. It is shown that many dyes supplied by different producers do not suit the purposes of quantitative cytochemical investigations. Before use the absorption spectra of the DNA-fuchsin must be taken using the standard object. The use of dyes possessing a multi-peak absorption curve should be avoided.
研究了DNA-品红的染色和吸收光谱对染料质量及席夫试剂制备方式的依赖性。结果表明,不同生产商提供的许多染料不适合定量细胞化学研究的目的。在使用前,必须使用标准物体测定DNA-品红的吸收光谱。应避免使用具有多峰吸收曲线的染料。