Suppr超能文献

[染料质量和席夫试剂制备方法对福尔根反应中染色强度及DNA-品红吸收光谱的影响]

[Effect of dye quality and the method of preparing a Schiff reagent on the staining intensity and DNA-fuchsin absorption spectra in performing the Feulgen reaction].

作者信息

Magakian Iu A, Avetisian A S, Karalova E M

出版信息

Tsitologiia. 1985 Sep;27(9):1078-85.

PMID:2414874
Abstract

Dependence of the staining and absorption spectra of DNA-fuchsin on the dye quality and modes of preparation of the Schiff-reagent was studied. It is shown that many dyes supplied by different producers do not suit the purposes of quantitative cytochemical investigations. Before use the absorption spectra of the DNA-fuchsin must be taken using the standard object. The use of dyes possessing a multi-peak absorption curve should be avoided.

摘要

研究了DNA-品红的染色和吸收光谱对染料质量及席夫试剂制备方式的依赖性。结果表明,不同生产商提供的许多染料不适合定量细胞化学研究的目的。在使用前,必须使用标准物体测定DNA-品红的吸收光谱。应避免使用具有多峰吸收曲线的染料。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验