Ye Z, Pal R, Ogden J R, Snyder R M, Wagner R R
Virology. 1985 Jun;143(2):657-62. doi: 10.1016/0042-6822(85)90408-8.
Of 33 hybridomas raised by immunization of BALB/c mice with the matrix (M) protein of the New Jersey serotype of vesicular stomatitis virus (VSV), 17 secreted monoclonal antibodies (mAb) of the IgG isotype and, unexpectedly, 16 of the IgM isotype. All these monoclonal antibodies bound strongly to VSV-New Jersey M protein by ELISA, immunoprecipitation, and immunoblotting assays, but exhibited only slight or no cross-reactivity with the M protein of VSV-Indiana. Four antigenic determinants of VSV-New Jersey M protein could be identified by competitive binding of 125I-labeled monoclonal antibodies but three of these epitopes exhibited partial overlap. Monoclonal antibodies to two epitopes reversed the inhibitory effect of M protein on in vitro transcription of VSV-New Jersey ribonucleoprotein. However, monoclonal antibodies to the other two epitopes had little effect on M-protein transcription inhibition but actually increased significantly the transcriptional inhibitory effect of M protein under certain experimental conditions. Monoclonal antibodies to all four epitopes reacted strongly with the M protein of the tsC1 mutant of VSV-New Jersey which is restricted in transcription inhibition.
用水疱性口炎病毒（VSV）新泽西血清型的基质（M）蛋白免疫BALB/c小鼠，产生了33个杂交瘤，其中17个分泌IgG同种型的单克隆抗体（mAb），出乎意料的是，16个分泌IgM同种型的单克隆抗体。通过ELISA、免疫沉淀和免疫印迹分析，所有这些单克隆抗体都与VSV-新泽西M蛋白强烈结合，但与VSV-印第安纳州的M蛋白仅表现出轻微或无交叉反应性。通过125I标记的单克隆抗体的竞争性结合可鉴定出VSV-新泽西M蛋白的四个抗原决定簇，但其中三个表位表现出部分重叠。针对两个表位的单克隆抗体可逆转M蛋白对VSV-新泽西核糖核蛋白体外转录的抑制作用。然而，针对其他两个表位的单克隆抗体对M蛋白转录抑制作用影响不大，但在某些实验条件下实际上显著增强了M蛋白的转录抑制作用。针对所有四个表位的单克隆抗体都与转录受限制的VSV-新泽西tsC1突变体的M蛋白强烈反应。
