Distribution of negative charges of rat brain synaptosomes established by means of protamine-ferritin conjugate.

The negative electric charges on the synaptosomes from rat cerebral cortex were studied by means of protamine-ferritin conjugate. The synaptic vesicles in some synaptosomes were heavily labelled with the positively charged conjugate. The synaptosomal membranes including presynaptic and postsynaptic membranes were also stained but in a lesser degree. It was established that the major dense line and the intraperiod line of myelin contaminants were labelled, too.