Arima T, Uemura T, Yamamoto T
Acta Otolaryngol. 1985 Sep-Oct;100(3-4):194-200. doi: 10.3109/00016488509104781.
The structural features of the basal lamina in Reissner's membrane were examined electron microscopically using tannic acid fixation, poly-cationic staining (ruthenium red), and mono-cationic staining (safranin O). Tannic acid fixation revealed 5-10-nm filaments which ran perpendicularly between the lamina densa and the cytoplasmic surface of squamous epithelial cells. Ruthenium red staining showed a lattice-like arrangement of anionic sites on both sides of the lamina densa. Safranin O staining revealed filamentous networks at the same sites. These structural features of the basal lamina in the Reissner's membrane were very similar to those of the glomerular and the alveolar basement membrane. Therefore, the possibility that anionic sites of Reissner's membrane may play the role of a size and charge-selective barrier between the scala media and the scala vestibuli, in much the same way that the glomerular basement membrane plays the same role of barrier, must be taken into account.
利用单宁酸固定、聚阳离子染色（钌红）和单阳离子染色（番红O），通过电子显微镜检查了Reissner膜中基底层的结构特征。单宁酸固定显示出5-10纳米的细丝，这些细丝在致密层和鳞状上皮细胞的细胞质表面之间垂直排列。钌红染色显示致密层两侧存在阴离子位点的晶格状排列。番红O染色显示相同部位有丝状网络。Reissner膜中基底层的这些结构特征与肾小球和肺泡基底膜的结构特征非常相似。因此，必须考虑到Reissner膜的阴离子位点可能在中阶和前庭阶之间起到大小和电荷选择性屏障的作用，其方式与肾小球基底膜起到相同的屏障作用非常相似。
