Saito H, Bourinbaiar A, Ginsburg M, Minato K, Ceresi E, Yamada K, Machover D, Bréard J, Mathé G
Blood. 1985 Dec;66(6):1233-40.
A human eosinophilic leukemia cell line, designated as EoL, was established from the peripheral blood of a patient with Philadelphia chromosome-negative eosinophilic leukemia (EL). The EoL cell line grows in single cell suspension with a doubling time of 48 hours for about one year. The reactivity of these cells was tested with a panel of monoclonal antibodies; they were found to express surface IA antigen, myeloid antigen (IF10, MY9) and membrane receptors for interleukin 2 (IL-2, Tac antigen). Under standard culture conditions, a small percentage of cells having more typical eosinophilic characteristics was present. These cells had cytoplasmic granules and were positive for Luxol-fast-blue and eosinophil peroxidase. Under culture conditions to induce the maturation of myeloid cells, such as alkaline medium or addition of dimethyl sulfoxide (DMSO), the frequency of cells with typical eosinophilic features increased to about 40%. In addition, cytogenetic studies showed that cultured cells and original leukemic blasts presented similar chromosome abnormalities. EoL seems to be a unique leukemic line committed to the eosinophilic lineage and can provide a useful in vitro model for the study of malignant eosinophilic properties.
一种人类嗜酸性粒细胞白血病细胞系，命名为EoL，是从一名费城染色体阴性嗜酸性粒细胞白血病（EL）患者的外周血中建立的。EoL细胞系以单细胞悬液形式生长，倍增时间为48小时，持续约一年。用一组单克隆抗体检测了这些细胞的反应性；发现它们表达表面IA抗原、髓系抗原（IF10、MY9）和白细胞介素2的膜受体（IL-2，Tac抗原）。在标准培养条件下，存在一小部分具有更典型嗜酸性粒细胞特征的细胞。这些细胞有细胞质颗粒，对卢氏固蓝和嗜酸性粒细胞过氧化物酶呈阳性。在诱导髓系细胞成熟的培养条件下，如碱性培养基或添加二甲基亚砜（DMSO），具有典型嗜酸性粒细胞特征的细胞频率增加到约40%。此外，细胞遗传学研究表明，培养的细胞和原始白血病母细胞呈现相似的染色体异常。EoL似乎是一种独特的致力于嗜酸性粒细胞谱系的白血病细胞系，可为研究恶性嗜酸性粒细胞特性提供有用的体外模型。
