De Braekeleer M, Keushnig M, Lin C C
Can J Genet Cytol. 1985 Oct;27(5):622-5. doi: 10.1139/g85-093.
A simple technique for synchronization of human lymphocyte cultures with fluorodeoxyuridine (FudR) is presented. The S-phase block induced by the FudR is released by simultaneous exposure to 5-bromodeoxyuridine (BrdU) and Hoechst 33258 or by thymidine and Hoechst 33258. This method provides a high mitotic index with high percentage of prometaphase chromosomes. This simple method is highly advantageous and easy to utilize in clinical cytogenetics.
本文介绍了一种使用氟脱氧尿苷（FudR）同步人淋巴细胞培养的简单技术。FudR诱导的S期阻滞可通过同时暴露于5-溴脱氧尿苷（BrdU）和Hoechst 33258或胸苷和Hoechst 33258来解除。该方法可提供高有丝分裂指数和高比例的早中期染色体。这种简单方法具有很大优势，且易于在临床细胞遗传学中应用。
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