Friedman H S, Schold S C, Bigner D D
Cancer Res. 1986 Jan;46(1):224-8.
The continuous human medulloblastoma cell line TE-671 was grown as s.c. and intracranial xenografts in athymic nude mice. Tumor-bearing animals were treated with chemotherapeutic agents at the 10% lethal dose; s.c. xenografts were sensitive to melphalan, 1-(2-chloroethyl)-3-(2,6-dioxo-1-piperidyl)-1-nitrosourea, and 5-azacytidine. No consistent response could be demonstrated to 9-beta-D-arabinofuranosyl-2-fluoroadenine 5'-monophosphate, and no response to methylglyoxal bis(guanyl hydrazone), N-trifluoroacetyl adriamycin-14-valerate, or to 1-beta-D-arabinofuranosylcytosine was observed. Melphalan produced a significant (P = less than or equal to 0.007) increase in the median survival of mice bearing intracranial xenografts, whereas no response was seen to 1-(2-chloroethyl)-3-(2,6-dioxo-1-piperidyl)-1-nitrosourea or 5-azacytidine. This model will allow analysis of the chemotherapeutic profile of human medulloblastoma, and provides a means to differentiate cellular sensitivity and resistance from drug access to the intracranial site.
人髓母细胞瘤连续细胞系TE - 671在无胸腺裸鼠体内作为皮下和颅内异种移植物生长。荷瘤动物接受10%致死剂量的化疗药物治疗；皮下异种移植物对美法仑、1-(2 -氯乙基)-3-(2,6 -二氧代-1 -哌啶基)-1 -亚硝基脲和5 -氮杂胞苷敏感。对9 -β - D -阿拉伯呋喃糖基-2 -氟腺嘌呤5'-单磷酸未显示出一致的反应，对甲基乙二醛双(胍腙)、N -三氟乙酰阿霉素-14 -戊酸酯或1 -β - D -阿拉伯呋喃糖基胞嘧啶未观察到反应。美法仑使颅内异种移植小鼠的中位生存期显著增加（P≤0.007），而对1-(2 -氯乙基)-3-(2,6 -二氧代-1 -哌啶基)-1 -亚硝基脲或5 -氮杂胞苷未见反应。该模型将有助于分析人髓母细胞瘤的化疗特征，并提供一种方法来区分细胞敏感性和耐药性与药物进入颅内部位的情况。
