Pankov R G, Uschewa A A, Tasheva B T, Petrov P T, Markov G G
Cell Biol Int Rep. 1985 Nov;9(11):1003-11. doi: 10.1016/0309-1651(85)90066-9.
A dominating protein fraction (p45) having molecular weight of 45000 and pI 5.45 was found in the intermediate filaments pellet obtained from rat liver besides the present cytokeratins. Peptide mapping and radioimmunological assays with antibodies against this protein and muscle actin proved that the p45 protein belongs to the actin group. Immunoelectron microscopy revealed that this protein is located on the liver intermediate filaments. By melting of the cytokeratin complexes in urea it was established that p45 protein is complexed with the low molecular weight cytokeratin.
除了现有的细胞角蛋白外，在从大鼠肝脏获得的中间丝沉淀中发现了一种主要的蛋白质组分（p45），其分子量为45000，等电点为5.45。用针对该蛋白质和肌肉肌动蛋白的抗体进行肽图谱分析和放射免疫测定证明，p45蛋白属于肌动蛋白组。免疫电子显微镜显示该蛋白质位于肝脏中间丝上。通过在尿素中溶解细胞角蛋白复合物，确定p45蛋白与低分子量细胞角蛋白复合。
