Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jan 24;118:782-6. doi: 10.1016/j.saa.2013.07.081. Epub 2013 Aug 6.
Herein, the effect of amino acid Glutathione (GSH) on Peroxyoxalate Chemiluminescence was studied for the first time. Hypericin (HYP) was employed as the efficient fluorophore. The investigated parameters included rise and fall rate constant for the chemiluminescence burst, theoretical and experimental maximum intensity, the time-needed to reach maximum intensity and the total light yield emission which theoretically was evaluated using the pooled intermediate model by a computerized non-linear least-squares curve fitting program (KINFIT). Furthermore, based on observed quenching effect of GSH, the Stern-Volmer plot in quencher concentration range of 2.8×10(-6) to 3.4×10(-5)M with KQ value of 1.59×10(4) was calculated. The bimolecular quenching rate constant (Kq) was also estimated about 2.8×10(12) and M(-1) S(-1). Moreover the system was applied successfully to determine glutathione in biological samples.
本文首次研究了氨基酸谷胱甘肽（GSH）对过氧草酸化学发光的影响。金丝桃素（HYP）被用作有效的荧光团。研究的参数包括化学发光爆发的上升和下降速率常数、理论和实验最大强度、达到最大强度所需的时间和总光产额发射，这是通过计算机非线性最小二乘曲线拟合程序（KINFIT）使用汇集的中间模型理论上评估的。此外，基于观察到的 GSH 猝灭效应，在 2.8×10(-6)至 3.4×10(-5)M 的猝灭剂浓度范围内计算了 Stern-Volmer 图，KQ 值为 1.59×10(4)。双分子猝灭速率常数（Kq）也估计约为 2.8×10(12)和 M(-1) S(-1)。此外，该系统成功应用于生物样品中谷胱甘肽的测定。
