Department of Chemistry, Isfahan University of Technology, Isfahan, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Oct 15;81(1):679-83. doi: 10.1016/j.saa.2011.07.001. Epub 2011 Jul 7.
A novel method for simple and sensitive determination of glucose based on the peroxyoxalate chemiluminescence (PO-CL) detection of enzymatically generated H(2)O(2) was investigated. Influence of various experimental parameters on glucose sensing, including the action time of the enzyme, solution pH, interferents and the concentration of CL reagents was investigated. Under the optimum condition, the linear response range of glucose was found to be 2.50×10(-6) to 1.75×10(-4) mol/L, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) was 1.10×10(-6) mol/L. The present method has been used to determine the glucose concentrations in real serum and urine samples with satisfactory results.
一种基于过氧草酸酯化学发光（PO-CL）检测酶促生成的 H(2)O(2)来简单灵敏地测定葡萄糖的新方法进行了研究。考察了各种实验参数对葡萄糖传感的影响，包括酶的作用时间、溶液 pH 值、干扰物和 CL 试剂的浓度。在最佳条件下，发现葡萄糖的线性响应范围为 2.50×10(-6) 至 1.75×10(-4) mol/L，检测限（定义为信噪比为 3 时可检测到的浓度）为 1.10×10(-6) mol/L。该方法已用于测定实际血清和尿液样本中的葡萄糖浓度，结果令人满意。
