Acta Pharm. 2013 Sep;63(3):419-26. doi: 10.2478/acph-2013-0030.
Abstract A simple RP HPLC method for quantification of betamethasone dipropionate (BDP) in gingival crevicular fluid (GCF) has been developed and validated. GCF represents a valuable matrix for therapeutic monitoring of drugs used in the treatment of periodontal disease. The proposed method involves single step extraction for sample preparation. The calibration curve for BDP was linear over the concentration range of 0.10-2.00 μg mL⁻¹ (R² = 0.9971). RSD values of intra- and inter-day precision ranged 2.2-4.5 and 1.6-5.7 %, while accuracy values were higher than 96.6 and 97.0 %, respectively. The described method can be successfully applied for determination of betamethasone concentrations in GCF obtained from patients with chronic periodontitis after local treatment with BDP cream 0.5 mg g⁻¹.
摘要 建立并验证了一种简单的反相高效液相色谱法，用于定量龈沟液（GCF）中的倍他米松二丙酸酯（BDP）。GCF 是监测牙周病治疗中所用药物的治疗药物监测的有价值的基质。该方法涉及用于样品制备的单步提取。BDP 的校准曲线在 0.10-2.00 μg mL⁻¹ 的浓度范围内呈线性（R² = 0.9971）。日内和日间精密度的 RSD 值分别为 2.2-4.5%和 1.6-5.7%，而准确度值均高于 96.6%和 97.0%。该方法可成功应用于测定局部使用 0.5 mg g⁻¹ BDP 乳膏治疗慢性牙周炎患者的 GCF 中的倍他米松浓度。
