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分裂间期新合成的RNA从细胞核和核仁向细胞质转运活性的变化。

Changes in the activity of transport of newly synthesized RNA from nucleus and nucleolus into cytoplasm during interphase.

作者信息

Kwiatkowska M, Maszewski J

出版信息

Folia Histochem Cytobiol. 1985;23(3):127-34.

PMID:2415407
Abstract

In antheridial filaments of Chara vulgaris given a 20 min incubation in 3H-uridine and a 40 min period of postincubation the radioactivity of extranucleolar nucleoplasm was found not altered, the radioactivity of nucleoli decreased by some 50%, and the radioactivity of cytoplasm increased by about 50%, as compared with the values obtained from preparations fixed immediately after labelling. During the periods characterized by an enhanced transcriptional activity of nucleoli, i.e. in middle stages of S and G2 phases, the most intensive RNA migration from nucleolus into the cytoplasm takes place. By the end of interphase the rate of the above process becomes slower, thus leading to accumulation of an intranucleolar pool of rRNA reserved for the sake of daughter cells.

摘要

在普通轮藻的雄器丝中,用3H-尿苷温育20分钟并进行40分钟的温育后,发现核仁外核质的放射性未改变,核仁的放射性降低了约50%,细胞质的放射性增加了约50%,与标记后立即固定的制剂所获得的值相比。在核仁转录活性增强的时期,即在S期和G2期的中期,RNA从核仁向细胞质的迁移最为强烈。到间期结束时,上述过程的速率变慢,从而导致为子细胞保留的核仁内rRNA池的积累。

相似文献

1
Changes in the activity of transport of newly synthesized RNA from nucleus and nucleolus into cytoplasm during interphase.分裂间期新合成的RNA从细胞核和核仁向细胞质转运活性的变化。
Folia Histochem Cytobiol. 1985;23(3):127-34.
2
Functional and structural heterogeneity of nucleoli: the dependence of the activity of transport of newly synthesized rRNA on nucleolar size and phase of the cell cycle.核仁的功能和结构异质性:新合成的rRNA转运活性对核仁大小和细胞周期阶段的依赖性。
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3
Nucleolar size and the activity of rRNA transport in the course of morphogenetic reduction of cell dimensions.细胞尺寸形态发生性减小过程中的核仁大小及核糖体RNA转运活性
Folia Histochem Cytobiol. 1985;23(3):135-44.
4
Changes in the activity of RNA polymerase detected in situ and the intensity of 3H uridine incorporation into the nucleolus and the nucleus of interphase cells in antheridial filaments of Chara vulgaris L.在原位检测到的RNA聚合酶活性变化以及3H尿苷掺入普通轮藻雄器丝间期细胞的核仁和细胞核的强度。
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5
Number, size, and transcriptional activity of nucleoli during different periods of interphase in antheridial filaments of Chara vulgaris L.普通轮藻雄器丝细胞间期不同阶段核仁的数量、大小及转录活性
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6
Enhanced rRNA transport from nucleoli into cytoplasm at G1/S, S/G2, and G2/M transitory points of the cell cycle in root meristem of Helianthus annuus L.在向日葵根分生组织细胞周期的G1/S、S/G2和G2/M过渡点,增强核糖体RNA从核仁向细胞质的转运
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The lack of correlation between the rate of rRNA transport from nucleoli into cytoplasm and the duration of G1 and G2 phases in root meristem cells.核糖体RNA从核仁转运到细胞质的速率与根分生组织细胞中G1期和G2期持续时间之间缺乏相关性。
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Ultrastructural localization of transcription sites and of RNA distribution during the cell cycle of synchronized CHO cells.
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[Study of incorporation of uridine-3H in the nucleus in the Amphidinium carteri, Dinoflagellata].
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