Huang Haiyan, Xu Xiaoguang, Yao Chen, Cai Ming, Qian Yeyong, Wang Xinying, Shi Bingyi
Organ Transplant Institute, Chinese PLA 309th Hospital, Beijing 100091, P.R. China.
Mol Med Rep. 2014 Jan;9(1):45-50. doi: 10.3892/mmr.2013.1753. Epub 2013 Oct 23.
The early diagnosis of acute rejection is crucial for graft survival after kidney transplantation. The interferon-γ (IFNγ)-CXCR3-chemokine-dependent inflammatory loop plays a pivotal role in the recruitment of T lymphocytes during acute rejection. Previously published studies have typically focused on the CXCR3 receptor rather than on its ligands. In the present study, we used Luminex assays to detect the levels of CXCR3 ligands, monokine induced by IFNγ (MIG), IFN-induced protein 10 (IP-10) and IFN-induced T‑cell chemoattractant (I-TAC), in the serum of renal allograft recipients. According to a renal biopsy performed one month after kidney transplantation, 32 recipients were diagnosed with T cell-mediated acute rejection and 38 patients were evaluated as stable. Serum was collected after the diagnosis of acute rejection or one month after transplantation. The concentrations of MIG (median, 4,271 pg/ml), IP-10 (median, 686.7 pg/ml) and I-TAC (median, 44.32 pg/ml) in the serum during an acute rejection episode were significantly higher compared with those of the stable patients (MIG, P=0.0002; IP-10, P=0.0001; I-TAC, P=0.0103; vs. the stable function group, P<0.05). Based on the receiver-operating characteristic (ROC) curve, the joint detection of MIG, IP-10 and I-TAC in the serum using Luminex analysis may constitute a non-invasive and efficient method for the early prediction of T cell-mediated acute rejection following kidney transplantation.
急性排斥反应的早期诊断对于肾移植后移植物存活至关重要。干扰素-γ（IFNγ）-CXCR3-趋化因子依赖性炎症环路在急性排斥反应期间T淋巴细胞的募集中起关键作用。先前发表的研究通常聚焦于CXCR3受体而非其配体。在本研究中，我们使用Luminex检测法来检测肾移植受者血清中CXCR3配体、IFNγ诱导的单核因子（MIG）、IFN诱导蛋白10（IP-10）和IFN诱导的T细胞趋化因子（I-TAC）的水平。根据肾移植后1个月进行的肾活检，32例受者被诊断为T细胞介导的急性排斥反应，38例患者被评估为病情稳定。在诊断急性排斥反应后或移植后1个月采集血清。与病情稳定的患者相比，急性排斥反应发作期间血清中MIG（中位数，4271 pg/ml）、IP-10（中位数，686.7 pg/ml）和I-TAC（中位数，44.32 pg/ml）的浓度显著更高（MIG，P=0.0002；IP-10，P=0.0001；I-TAC，P=0.0103；与功能稳定组相比，P<0.05）。基于受试者工作特征（ROC）曲线，使用Luminex分析联合检测血清中的MIG、IP-10和I-TAC可能构成一种非侵入性且高效的方法，用于早期预测肾移植后T细胞介导的急性排斥反应。
