Holthöfer H, Virtanen I, Törnroth T, Miettinen A
Histochem J. 1985 Aug;17(8):905-12. doi: 10.1007/BF01004185.
Different lectins were used to study frozen sections of kidney samples showing alterations in routine immunofluorescence studies. Arachis hypogaea agglutinin (peanut lectin, PNA), lacking binding sites in normal glomeruli, bound to the glomeruli in two of the five samples studied, giving a granular fluorescence pattern. Concomitantly with the appearance of PNA-binding, binding sites for wheat germ agglutinin (WGA) appeared to be lost at glomeruli. Furthermore, changes in the expression of glomerular binding sites for Wistaria floribunda (WFA), Helix pomatia (HPA) and Dolichos biflorus (DBA) agglutinins could be seen in the kidneys studied, whereas the binding sites for Ulex europaeus agglutin (UEA I) in vascular endothelia seemed to be unaltered. The results show that kidney specimens presenting changes in routine immunofluorescence studies may also present altered binding for certain lectins. On this basis we propose that certain lectins may aid in characterizing these changes and are thus of potential use in studying diseased kidneys.
使用不同的凝集素研究肾脏样本的冰冻切片，这些样本在常规免疫荧光研究中呈现出改变。花生凝集素（花生凝集素，PNA）在正常肾小球中缺乏结合位点，但在所研究的五个样本中的两个样本的肾小球中结合，呈现出颗粒状荧光模式。与PNA结合的出现同时，小麦胚芽凝集素（WGA）的结合位点在肾小球处似乎消失。此外，在所研究的肾脏中可以看到紫藤（WFA）、苹果蜗牛（HPA）和双花扁豆（DBA）凝集素的肾小球结合位点表达发生变化，而血管内皮中的欧洲荆豆凝集素（UEA I）结合位点似乎未改变。结果表明，在常规免疫荧光研究中呈现变化的肾脏标本，对于某些凝集素的结合也可能发生改变。在此基础上，我们提出某些凝集素可能有助于表征这些变化，因此在研究患病肾脏方面具有潜在用途。
