Faraggiana T, Malchiodi F, Prado A, Churg J
J Histochem Cytochem. 1982 May;30(5):451-8. doi: 10.1177/30.5.7077075.
The carbohydrate histochemistry of normal human kidney has been investigated by the use of four peroxidase-labeled lectins at the light and electron microscopic level. The results show that the lectin of Lotus tetragonolobus, specific for l-fucose, binds exclusively to the proximal convoluted tubules of the nephron. While peanut and soybean lectins, specific for D-galactose and N-acetyl-D-galactosamine, respectively, are confirmed to the collecting ducts, wheat germ lectin, specific for sialic acid and N-acetyl-D-glucosamine, stains several parenchymal structures, including the glomerular capillary wall, particularly its podocyte cell coat. Sialidase digestion reveals strong binding sites for peanut and soybean lectin in the glomeruli. At the ultrastructural level most of the binding is shown to be on the podocyte surface and within the lamina rara externa of the basement membrane. The technique represents a potentially very useful tool for the study of various pathological states in the kidney.
利用四种过氧化物酶标记的凝集素，在光学显微镜和电子显微镜水平上对正常人体肾脏的碳水化合物组织化学进行了研究。结果表明，对L-岩藻糖具有特异性的四角豆凝集素仅与肾单位的近端曲管结合。而分别对D-半乳糖和N-乙酰-D-半乳糖胺具有特异性的花生凝集素和大豆凝集素则在集合管中得到证实，对唾液酸和N-乙酰-D-葡糖胺具有特异性的麦胚凝集素可使包括肾小球毛细血管壁，尤其是其足细胞细胞衣在内的几种实质结构着色。唾液酸酶消化显示肾小球中存在花生凝集素和大豆凝集素的强结合位点。在超微结构水平上，大多数结合显示在足细胞表面和基底膜的外疏松层内。该技术是研究肾脏各种病理状态的一种潜在非常有用的工具。
