通过两种具有生物活性代谢产物的植物促生根际细菌控制木豆枯萎病和促进其生长。

Fusarial wilt control and growth promotion of pigeon pea through bioactive metabolites produced by two plant growth promoting rhizobacteria.

机构信息

Biotechnology Division, North East Institute of Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Jorhat, 785006, Assam, India.

出版信息

World J Microbiol Biotechnol. 2014 Mar;30(3):1111-21. doi: 10.1007/s11274-013-1532-9. Epub 2013 Oct 24.

DOI:10.1007/s11274-013-1532-9

PMID:24154979

Abstract

The bioactive metabolites produced by two plant growth promoting rhizobacteria strains, a Pseudomonas aeruginosa strain RRLJ 04 and a Bacillus cereus strain BS 03, which showed growth promotion and disease control in pigeon pea against Fusarium udum, were isolated and screened for their efficacy to control fusarial wilt of pigeon pea under gnotobiotic and nursery condition. Bioactive metabolites viz., BM 1 and BM 2 from RRLJ 04 and BM 3 from BS 03 also showed in vitro antibiosis against F. udum. Seeds treated with 50 μl seed⁻¹ of BM 1, 30 μl seed⁻¹ of BM 2 and 70 μl seed⁻¹ of BM 3 and grown in pathogen infested soil showed suppression of wilt disease besides growth enhancement. Per cent disease control was 90 % with BM 2 application as compared to 87 and 83 %, respectively in BM 1 and BM 3 after 90 days of growth. BM 2 treated plants were more resistant to the pathogen as compared to the other fractions tested. Mycelial dry weight was found to be reduced on treatment with the bioactive metabolites. Formation of chlamydospore-like structures was observed in the pathogen mycelium treated with BM 3. The analytical studies confirmed that two of these metabolites are phenazine derivatives.

摘要

从表现出促进豇豆生长和防治镰孢菌根腐病的两株植物促生根际细菌（一株铜绿假单胞菌 RRLJ04 和一株蜡样芽胞杆菌 BS03）中分离和筛选了生物活性代谢产物，以评估它们在无菌和苗圃条件下防治豇豆花叶病毒的功效。RRLJ04 产生的生物活性代谢产物 BM1 和 BM2 以及 BS03 产生的 BM3 对 F.udum 也表现出体外抑菌活性。用 50 μl BM1、30 μl BM2 和 70 μl BM3 处理种子，然后在有病原菌的土壤中生长，除了促进生长外，还抑制了萎蔫病。与 BM1 和 BM3 相比，应用 BM2 处理后 90 天的萎蔫病防治率分别为 90%、87%和 83%。与其他测试的部分相比，用 BM2 处理的植物对病原体的抗性更强。用生物活性代谢产物处理后发现，菌丝干重降低。用 BM3 处理病原体菌丝后，观察到类似厚垣孢子的结构形成。分析研究证实，其中两种代谢产物是吩嗪衍生物。