Department of Craniofacial Development and Stem Cell Biology, Department of Orthodontics, King's College London, London, UK, SE1 9RT.
J Dent Res. 2013 Dec;92(12):1129-35. doi: 10.1177/0022034513510321. Epub 2013 Oct 23.
The tooth works as a functional unit with its surrounding bony socket, the alveolar bone. The growth of the tooth and alveolar bone is co-ordinated so that a studied distance always separates the 2, known as the tooth-bone interface (TBI). Lack of mineralization, a crucial feature of the TBI, creates the space for the developing tooth to grow and the soft tissues of the periodontium to develop. We have investigated the interactions between the tooth and its surrounding bone during development, focusing on the impact of the developing alveolar bone on the development of the mouse first molar (M1). During development, TRAP-positive osteoclasts are found to line the TBI as bone starts to be deposited around the tooth, removing the bone as the tooth expands. An enhancement of osteoclastogenesis through RANK-RANKL signaling results in an expansion of the TBI, showing that osteoclasts are essential for defining the size of this region. Isolation of the M1 from the surrounding mesenchyme and alveolar bone leads to an expansion of the tooth germ, driven by increased proliferation, indicating that, during normal development, the growth of the tooth germ is constrained by the surrounding tissues.
牙齿与其周围的骨性牙槽骨（牙槽骨）一起作为功能单位发挥作用。牙齿和牙槽骨的生长是协调的，因此始终保持着研究人员确定的特定距离，这个距离被称为牙骨界面（TBI）。TBI 的一个关键特征是缺乏矿化，这为正在发育的牙齿的生长和牙周软组织的发育创造了空间。我们研究了牙齿与其周围骨骼在发育过程中的相互作用，重点研究了发育中的牙槽骨对小鼠第一磨牙（M1）发育的影响。在发育过程中，当牙周围开始沉积骨时，TRAP 阳性破骨细胞被发现排列在 TBI 周围，随着牙齿的扩张去除骨。通过 RANK-RANKL 信号增强破骨细胞生成会导致 TBI 扩大，表明破骨细胞对于确定该区域的大小至关重要。将 M1 从周围的间充质和牙槽骨中分离出来会导致牙胚的扩大，这是由增殖增加驱动的，表明在正常发育过程中，牙胚的生长受到周围组织的限制。
