Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
Biofactors. 2013 Sep-Oct;39(5):542-51. doi: 10.1002/biof.1097. Epub 2013 Apr 5.
The present investigation pertains to changes in substance P-like immunoreactive (SP-LI) nerve structures of the enteric nervous system (ENS) in the porcine descending colon, caused by chemically-induced inflammation and nerve injury (axotomy). The distribution pattern of SP-LI structures was studied using the double immunofluorescence technique in the myenteric (MP), outer submucous (OSP) and inner submucous (ISP) plexuses, as well as in the circular muscle and mucosal layers. Under physiological conditions, SP-LI neurons have been shown to constitute 4.13 ± 0.24%, 3.36 ± 0.26%, and 7.92 ± 0.16% in the MP, OSP, and ISP, respectively. Changes in SP-immunoreactivity depended on the pathological factor studied. The numbers of the SP-LI perikarya amounted to 7.89 ± 0.34, 5.56 ± 0.30, and 19.96 ± 0.57 in chemically-induced colitis, and 4.28 ± 0.13%, 7.18 ± 20%, and 11.62 ± 0.48% after axotomy in MP, OSP, and ISP, respectively. The both studied processes generally resulted in an increase in the number of SP-LI nerve fibers in the circular muscle and mucosal layers. The obtained results suggest that SP-LI nerve structures of the ENS may participate in various pathological processes in the porcine descending colon and exact functions of SP probably depend on the type of the pathological factor.
本研究旨在探讨化学诱导的炎症和神经损伤（轴突切断）对猪降结肠肠神经系统（ENS）中 P 物质样免疫反应（SP-LI）神经结构的影响。采用双重免疫荧光技术，研究了 SP-LI 结构在肌间神经丛（MP）、外黏膜下神经丛（OSP）和内黏膜下神经丛（ISP）以及环形肌和黏膜层中的分布模式。在生理条件下，MP、OSP 和 ISP 中的 SP-LI 神经元分别构成 4.13±0.24%、3.36±0.26%和 7.92±0.16%。SP 免疫反应的变化取决于所研究的病理因素。在化学诱导的结肠炎中，SP-LI 神经元的数量分别为 7.89±0.34、5.56±0.30 和 19.96±0.57，而在 MP、OSP 和 ISP 中，轴突切断后的数量分别为 4.28±0.13%、7.18±20%和 11.62±0.48%。这两种研究过程通常导致环形肌和黏膜层中 SP-LI 神经纤维数量增加。研究结果表明，ENS 的 SP-LI 神经结构可能参与猪降结肠的各种病理过程，而 SP 的确切功能可能取决于病理因素的类型。
