Furukawa K, Mattes M J, Lloyd K O
J Immunol. 1985 Dec;135(6):4090-4.
The differential reactivity of four mouse monoclonal antibodies (AbCB, AbHT29-36, AbM2, and AbS12) and Dolichos biflorus lectin with A1 and A2 erythrocytes was analyzed. Only AbS12 and D. biflorus lectin were able to preferentially agglutinate A1 erythrocytes. AbS12 is known to react only with short chain, unbranched structures (such as Aa-2 and Ab-2 glycolipids) and not with longer chains or with type 3 and type 4 structures. D. biflorus was shown to have a similar specificity by lectin staining of glycolipids separated by thin-layer chromatography. Analysis of the binding of radiolabeled AbCB and AbS12 to A1 and A2 erythrocytes by Scatchard analysis showed that, whereas the former antibody recognizes high-affinity sites on both A1 and A2 cells, AbS12 reacts with high-affinity sites only on A1 cells. Because A1 and A2 erythrocytes have a similar complement of short chain type 2 glycolipids, although in different amounts, it is suggested that AbS12 and D. biflorus lectin differentiate between the two cell types on the basis of quantitative, nonstructural features. This is in contrast to AbTH1, which reacts with a repetitive A epitope (type 3 A chain) and distinguishes between A1 and A2 cells based on the preferential expression of type 3 A chains in A1 erythrocytes. Thus, two views of A1/A2, i.e., qualitative vs quantitative are correct, depending on the properties of the reagent being used to distinguish between the two cell types.
分析了四种小鼠单克隆抗体（AbCB、AbHT29 - 36、AbM2和AbS12）以及双花扁豆凝集素与A1和A2红细胞的差异反应性。只有AbS12和双花扁豆凝集素能够优先凝集A1红细胞。已知AbS12仅与短链、无分支结构（如Aa - 2和Ab - 2糖脂）反应，而不与长链或3型和4型结构反应。通过薄层色谱分离的糖脂的凝集素染色显示双花扁豆具有相似的特异性。通过Scatchard分析对放射性标记的AbCB和AbS12与A1和A2红细胞的结合进行分析表明，前者抗体识别A1和A2细胞上的高亲和力位点，而AbS12仅与A1细胞上的高亲和力位点反应。由于A1和A2红细胞具有相似的短链2型糖脂互补物，尽管数量不同，提示AbS12和双花扁豆凝集素基于数量而非结构特征区分这两种细胞类型。这与AbTH1相反，AbTH1与重复的A表位（3型A链）反应，并基于A1红细胞中3型A链的优先表达区分A1和A2细胞。因此，根据用于区分这两种细胞类型的试剂的性质，A1/A2的两种观点，即定性与定量观点都是正确的。
