Nakajima T, Kogure T, Furukawa K
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Exp Clin Immunogenet. 1986;3(4):187-94.
Saline extract of the seeds of Falcata japonica has been shown to have hemagglutinating activity for saline red cells of group A, Cad positive and Tn. In titration the extract agglutinates more strongly A2 red cells than the extract of Dolichos biflorus. Because the extract of F. japonica has no ability to agglutinate A3 red cells, it could be used to distinguish the subgroups of A in comparison with the agglutinability of human anti-A serum and D. biflorus lectin. F. japonica extract agglutinates much more strongly with papain-treated A red cells, and papain-treated O and B red cells come to be agglutinated with the extract. The agglutination reactions of lectin of F. japonica with untreated A, and papain-treated A and O red cells are inhibited by A secretor saliva and A substances from human and hog gastric linings. N-Acetyl-D-galactosamine is a potent inhibitor of the hemagglutination reaction in monosaccharides tested. It is directed against terminal GalNAc structures either alpha- or beta-linked.
日本黄花棘豆种子的盐水提取物已被证明对A组、Cad阳性和Tn的盐水红细胞具有血凝活性。在滴定中，该提取物对A2红细胞的凝集作用比双花扁豆提取物更强。由于日本黄花棘豆提取物没有凝集A3红细胞的能力，与人类抗A血清和双花扁豆凝集素的凝集能力相比，它可用于区分A的亚组。日本黄花棘豆提取物与木瓜蛋白酶处理的A红细胞凝集作用更强，木瓜蛋白酶处理的O和B红细胞也会被该提取物凝集。日本黄花棘豆凝集素与未处理的A以及木瓜蛋白酶处理的A和O红细胞的凝集反应可被A分泌型唾液以及来自人和猪胃黏膜的A物质抑制。在测试的单糖中，N-乙酰-D-半乳糖胺是血凝反应的有效抑制剂。它针对的是α-或β-连接的末端GalNAc结构。
