Department of Neurosurgery, The Affiliated Hospital of Medical College, Qingdao University , Shandong Province , P. R. China.
Artif Cells Nanomed Biotechnol. 2014 Jun;42(3):186-91. doi: 10.3109/21691401.2013.794350. Epub 2013 Oct 25.
We report that adenovirus mediated TNF-related apoptosis-inducing ligand (TRAIL) influenced the cell growth and cell cycle in the glioma cells in vitro. After being infected with the Ad-sTRAIL, U251 cell growth was inhibited. The expression of sTRAIL was detected using immunofluorescence. The higher rate of apoptosis was demonstrated using short-term microculture tetrazoliun (MTT) assay and flow cytometry. The rate of Ad-sTRAIL-inducing U251 cell apoptosis was increased depending on the dosage and the time. The apoptosis of G0/G1 and S phase cells was more significant than that of the control groups. The growth and proliferation of U251 cell line was inhibited after the infection of Ad-sTRAIL. It is dose- and time dependent.
我们报告称，腺病毒介导的肿瘤坏死因子相关凋亡诱导配体（TRAIL）能够影响体外脑胶质瘤细胞的细胞生长和细胞周期。在感染 Ad-sTRAIL 后，U251 细胞的生长受到抑制。采用免疫荧光法检测 sTRAIL 的表达。通过短期微量培养四唑盐（MTT）测定和流式细胞术检测到更高的细胞凋亡率。Ad-sTRAIL 诱导 U251 细胞凋亡的比率随着剂量和时间的增加而增加。与对照组相比，G0/G1 和 S 期细胞的凋亡更为显著。Ad-sTRAIL 感染后 U251 细胞系的生长和增殖受到抑制，这与剂量和时间有关。
