Department of Occupational Health, Wuxi Center for Disease Control and Prevention , Wuxi , China and.
Toxicol Mech Methods. 2014 Feb;24(2):111-5. doi: 10.3109/15376516.2013.859195. Epub 2013 Nov 18.
GSTP1 is induced by lead, and thus serves as a biomarker of lead exposure. Lead exposure changes DNA methylation status.
We attempted to prove that the methylation of the GSTP1 promoter plays an important role in lead toxicity.
We conducted a case-control study of 53 workers from a battery plant and 53 age and sex matched healthy volunteers to determine whether the methylation level of the GSTP1 promoter is associated with the risk of lead poisoning. We employed methylation-specific PCR (MSP) in cell models to determine the relationship between the GSTP1 methylation level and lead exposure.
We found no association between GSTP1 methylation and lead exposure. The difference in methylation frequencies between the exposure group and the controls was not statistically significant (p = 0.401), and individuals with the methylated GSTP1 gene was not associated with the risk of lead poisoning (adjusted OR = 1.36, 95% CI, 0.22-8.24).
This study suggests that GSTP1 methylation is not involved in the early phase of lead toxicity. Further studies should be performed to detect the association between GSTP1 methylation and the risk of lead poisoning in later phases.
GSTP1 可被铅诱导，因此可作为铅暴露的生物标志物。铅暴露会改变 DNA 甲基化状态。
我们试图证明 GSTP1 启动子的甲基化在铅毒性中起重要作用。
我们对一家电池厂的 53 名工人和 53 名年龄和性别匹配的健康志愿者进行了病例对照研究，以确定 GSTP1 启动子的甲基化水平是否与铅中毒的风险相关。我们在细胞模型中采用甲基化特异性 PCR（MSP）来确定 GSTP1 甲基化水平与铅暴露之间的关系。
我们未发现 GSTP1 甲基化与铅暴露之间存在关联。暴露组和对照组之间的甲基化频率差异无统计学意义（p=0.401），并且具有甲基化 GSTP1 基因的个体与铅中毒的风险无关（调整后的 OR=1.36，95%CI，0.22-8.24）。
本研究提示 GSTP1 甲基化不参与铅毒性的早期阶段。应进一步开展研究以检测 GSTP1 甲基化与铅中毒后期风险之间的关联。
