非典型福氏志贺菌II:(3)4,7(8)的III型分泌系统表达增强

Enhanced Type III Secretion System Expression of Atypical Shigella flexneri II:(3)4,7(8).

作者信息

Hong Sahyun, Cha Injun, Kim Nan-Ok, Kim Seong-Han, Jung Kyung-Tae, Lee Je-Hee, Kim Dong-Wook, Park Mi-Sun, Kang Yeon-Ho

机构信息

Division of Enteric Bacterial Infections, Korea National Institute of Health, Osong, Korea.

出版信息

Osong Public Health Res Perspect. 2012 Dec;3(4):222-8. doi: 10.1016/j.phrp.2012.10.002.

DOI:10.1016/j.phrp.2012.10.002

PMID:24159518

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3747661/

Abstract

OBJECTIVES

We aimed at evaluating the virulence of atypical Shigella flexneri II:(3)4,7(8) by DNA microarray and invasion assay.

METHODS

We used a customized S. flexneri DNA microarray to analyze an atypical S. flexneri II:(3)4,7(8) gene expression profile and compared it with that of the S. flexneri 2b strain.

RESULTS

Approximately one-quarter of the atypical S. flexneri II:(3)4,7(8) strain genes showed significantly altered expression profiles; 344 genes were more than two-fold upregulated, and 442 genes were more than 0.5-fold downregulated. The upregulated genes were divided into the category of 21 clusters of orthologous groups (COGs), and the "not in COGs" category included 170 genes. This category had virulence plasmid genes, including the ipa-mxi-spa genes required for invasion of colorectal epithelium (type III secretion system). Quantitative reverse-transcription polymerase chain reaction results also showed the same pattern in two more atypical S. flexneri II:(3)4,7(8) strains. Atypical S. flexneri II:(3)4,7(8) showed four times increased invasion activity in Caco-2 cells than that of typical strains.

CONCLUSION

Our results provide the intracellularly regulated genes that may be important for adaptation and growth strategies of this atypical S. flexneri.

摘要

目的

我们旨在通过DNA微阵列和侵袭试验评估非典型福氏志贺菌II:(3)4,7(8)的毒力。

方法

我们使用定制的福氏志贺菌DNA微阵列分析非典型福氏志贺菌II:(3)4,7(8)的基因表达谱，并将其与福氏志贺菌2b菌株的基因表达谱进行比较。

结果

大约四分之一的非典型福氏志贺菌II:(3)4,7(8)菌株基因显示出明显改变的表达谱；344个基因上调超过两倍，442个基因下调超过0.5倍。上调的基因分为21个直系同源群（COG）类别，“不在COG中”的类别包括170个基因。该类别中有毒力质粒基因，包括侵袭结肠上皮所需的ipa-mxi-spa基因（III型分泌系统）。定量逆转录聚合酶链反应结果在另外两株非典型福氏志贺菌II:(3)4,7(8)菌株中也显示出相同的模式。非典型福氏志贺菌II:(3)4,7(8)在Caco-2细胞中的侵袭活性比典型菌株高四倍。