一种由单克隆抗体所界定、受人类1号染色体上一个基因控制的人类细胞表面抗原。

A human cell-surface antigen defined by a monoclonal antibody and controlled by a gene on human chromosome 1.

作者信息

Andrews P W, Knowles B B, Parkar M, Pym B, Stanley K, Goodfellow P N

出版信息

Ann Hum Genet. 1985 Jan;49(1):31-9. doi: 10.1111/j.1469-1809.1985.tb01673.x.

DOI:10.1111/j.1469-1809.1985.tb01673.x

Abstract

An antigen expressed by most human cells, but not erythrocytes, has been defined by a murine monoclonal antibody, TRA-2-10. This antigen is expressed on the surface of human-mouse somatic cell hybrids, and segregation analysis indicates that it is controlled by a gene located on human chromosome 1. From lysates of most human cells, surface-labelled with 125I, TRA-2-10 immunoprecipitates two polypeptides with molecular weights in the range of about 55 000 to 73 000 depending upon the cell line. Since the TRA-2-10 polypeptides from a fibroblast cell strain and a hepatoma cell line from one individual differ, we conclude that the observed difference in molecular weight has an epigenetic origin.

摘要

一种由大多数人类细胞而非红细胞表达的抗原已被一种鼠单克隆抗体TRA - 2 - 10所定义。这种抗原在人 - 鼠体细胞杂种的表面表达，并且分离分析表明它受位于人类1号染色体上的一个基因控制。从用¹²⁵I进行表面标记的大多数人类细胞裂解物中，TRA - 2 - 10免疫沉淀出两种分子量在约55000至73000范围内的多肽，这取决于细胞系。由于来自同一个体的成纤维细胞株和肝癌细胞系的TRA - 2 - 10多肽不同，我们得出结论，观察到的分子量差异具有表观遗传起源。