Schäfer R, Franklin R M
Eur J Biochem. 1975 Oct 1;58(1):81-5. doi: 10.1111/j.1432-1033.1975.tb02351.x.
A polymerase activity is associated with protein IV, a protein which is associated with the DNA in bacteriophage PM2. The native enzyme unit is probably a dimer. Manganese ions are required for the polymerisation reaction and there is a well-defined Mn2+ optimum at 2.5 mM. The pH optimum is at 8.1, the temperature optimum at 28 degrees C. The activity is a polynucleotide-pyrophosphorylating reaction in the presence of ribo- or deoxyribonucleoside triphosphates. The polymerisation reaction is stimulated in the presence of nuclei- acids or polynucleotides as effectors. The product is not covalently linked to the effector.
一种聚合酶活性与蛋白质IV相关，蛋白质IV是一种与噬菌体PM2中的DNA相关的蛋白质。天然酶单位可能是二聚体。聚合反应需要锰离子，在2.5 mM时存在明确的锰离子最佳浓度。最适pH为8.1，最适温度为28摄氏度。该活性是在核糖或脱氧核糖核苷三磷酸存在下的多核苷酸焦磷酸化反应。核酸或多核苷酸作为效应物存在时可刺激聚合反应。产物与效应物不是共价连接的。
