Shimokado K, Raines E W, Madtes D K, Barrett T B, Benditt E P, Ross R
Cell. 1985 Nov;43(1):277-86. doi: 10.1016/0092-8674(85)90033-9.
The macrophage has been suggested to be responsible for the connective tissue cell proliferation that accompanies most chronic inflammatory responses. One of the secretory products of activated macrophages is MDGF, a growth factor (or factors) for fibroblasts, 3T3 cells, smooth muscle, and vascular endothelium. This report demonstrates that a significant portion of the mitogenic activity for 3T3 cells secreted by cultured human alveolar and peritoneal macrophages is due to a molecule (or molecules) similar to platelet-derived growth factor (PDGF). Two size classes (approximately 37,000-39,000 and 12,000-17,000 daltons) of mitogenically active PDGF-like molecules are detected by two criteria--antigenic similarity with PDGF and ability to compete with 125I-PDGF for high-affinity binding to the PDGF receptor. The presence of mRNA for the B chain of PDGF is demonstrated by Northern analysis, and de novo synthesis of these molecules by activated macrophages is shown by immunoprecipitation of 35S-labeled proteins with anti-PDGF IgG.
巨噬细胞被认为是大多数慢性炎症反应中伴随的结缔组织细胞增殖的原因。活化巨噬细胞的分泌产物之一是MDGF，一种成纤维细胞、3T3细胞、平滑肌和血管内皮细胞的生长因子。本报告表明，培养的人肺泡巨噬细胞和腹腔巨噬细胞分泌的对3T3细胞有丝分裂活性的很大一部分是由于一种（或几种）类似于血小板衍生生长因子（PDGF）的分子。通过两个标准检测到两种有丝分裂活性的PDGF样分子大小类别（约37,000-39,000和12,000-17,000道尔顿）——与PDGF的抗原相似性以及与125I-PDGF竞争与PDGF受体高亲和力结合的能力。通过Northern分析证明了PDGF B链mRNA的存在，并且通过用抗PDGF IgG免疫沉淀35S标记的蛋白质表明活化巨噬细胞从头合成这些分子。
