Imai K, Kumasaka F, Kobayashi M, Takahashi Y, Takahashi E, Yamanaka H
Nihon Naibunpi Gakkai Zasshi. 1985 Aug 20;61(8):816-22. doi: 10.1507/endocrine1927.61.8_816.
The effect of sodium molybdate on the specific binding protein (SBP) of synthetic progestin 17 alpha-methyl-[3H]-promegestone (R5020) in the cytosol of the human prostate was studied. In a sucrose density gradient analysis, two R5020 SBP components at 4S and 7-8S were observed. It was apparent that the 4S component was reduced and the 7-8S component increased with the addition of 10mM sodium molybdate into the cytosol. Therefore, the molybdate enhancement degree on total SBP amount (4S plus 7-8S) was decided by the relationship between the decreasing rate at 4S and the increasing one at 7-8S. It was shown that the molybdate effect was time-dependent and was not related to the SBP state, whether it was bounded with steroid or not. Moreover, it was estimated that the molybdate effect was not related to phosphatase inhibition since R5020 SBP in SDG was not enhanced by the addition of sodium fluoride which was a phosphatase inhibitor. In this report, the possibility of the existence of the 7-8S forming factor in the human prostate and the relationship between it and sodium molybdate was also discussed through an experiment on a Sephadex G-25.
研究了钼酸钠对人前列腺细胞质中合成孕激素17α-甲基-[3H]-炔诺孕酮（R5020）特异性结合蛋白（SBP）的影响。在蔗糖密度梯度分析中，观察到4S和7-8S处有两种R5020 SBP成分。很明显，向细胞质中添加10mM钼酸钠后，4S成分减少，7-8S成分增加。因此，钼酸盐对总SBP量（4S加7-8S）的增强程度取决于4S处的减少速率与7-8S处的增加速率之间的关系。结果表明，钼酸盐的作用具有时间依赖性，且与SBP状态无关，无论其是否与类固醇结合。此外，据估计，钼酸盐的作用与磷酸酶抑制无关，因为在蔗糖密度梯度（SDG）中，添加磷酸酶抑制剂氟化钠并不能增强R5020 SBP。在本报告中，还通过葡聚糖凝胶G-25实验讨论了人前列腺中7-8S形成因子存在的可能性及其与钼酸钠的关系。
