Tripp Valerie T, McKenna Jaclyn R, Young Douglas D
Integrated Science Center, College of William and Mary, Williamsburg, VA, USA.
Methods Mol Biol. 2014;1095:27-58. doi: 10.1007/978-1-62703-703-7_3.
The therapeutic importance of microRNA (miRNA) regulation has recently been realized as these small, noncoding RNAs have been demonstrated to be involved with a plethora of diseases and disorders. Due to the complex miRNA maturation process, the expression of these important biomolecules can be manipulated at various stages of the pathway. This review examines both in vivo and in vitro mechanisms and assays that have been developed to regulate miRNA levels. Modulation of miRNA maturation can be accomplished via several therapeutic agents, including small molecules and oligonucleotides, in both specific and nonspecific fashions. Due to the relevance of miRNAs, these novel therapeutic approaches represent new tools for the treatment of various cancers and other deleterious disorders.
近年来，人们已经认识到微小RNA（miRNA）调控的治疗重要性，因为这些小的非编码RNA已被证明与多种疾病和病症有关。由于miRNA成熟过程复杂，这些重要生物分子的表达可在该途径的各个阶段进行调控。本文综述了已开发出的用于调控miRNA水平的体内和体外机制及检测方法。miRNA成熟的调控可通过多种治疗剂实现，包括小分子和寡核苷酸，方式有特异性和非特异性两种。鉴于miRNA的相关性，这些新型治疗方法为治疗各种癌症和其他有害病症提供了新工具。
