微小RNA成熟机制组分的表达谱分析

Expression profiling of components of the miRNA maturation machinery.

作者信息

Sand Michael, Skrygan Marina

机构信息

Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Bochum, Germany.

出版信息

Methods Mol Biol. 2014;1095:61-71. doi: 10.1007/978-1-62703-703-7_4.

DOI:10.1007/978-1-62703-703-7_4

PMID:24166302

Abstract

The quantitative real-time polymerase chain reaction (qRT-PCR) is a valuable and well-proven technique used to investigate the expression level of multiple components of the microRNA (miRNA) maturation machinery. Here, we describe how to determine the messenger RNA expression levels of components of the miRNA machinery starting from the isolation of the RNA from a tissue biopsy to performance of the qRT-PCR.

摘要

定量实时聚合酶链反应（qRT-PCR）是一种有价值且经过充分验证的技术，用于研究微小RNA（miRNA）成熟机制中多个组分的表达水平。在此，我们描述了如何从组织活检中分离RNA开始，到进行qRT-PCR，来确定miRNA机制组分的信使RNA表达水平。