Asem E, Marrone B L, Hertelendy F
Gen Comp Endocrinol. 1985 Dec;60(3):353-60. doi: 10.1016/0016-6480(85)90068-1.
The influence of follicular maturation on steroidogenesis and steroid metabolism by isolated Japanese quail granulosa and theca cells was examined. When stimulated with LH, granulosa cells of the largest follicle (F1) responded with a sixfold increase over unstimulated progesterone levels, whereas progesterone production in cells of F3 less than doubled even when maximally stimulated. Forskolin stimulated progesterone synthesis in both F1 and F3 granulosa cells, but its effect was less pronounced than that of LH. Furthermore, F1 cells metabolized 25-hydroxycholesterol to a greater extent than did F3 cells. There was no appreciable metabolism of [3H]progesterone by granulosa cells. Theca cells from the smaller follicles (F3-F5) responded to LH stimulation with greater estrogen and androstenedione production than theca cells from F1. [3H]Progesterone was metabolized mainly to androstenedione in theca cells. Thus, the overall pattern of in vitro steroidogenesis in quail granulosa cells is similar to that described for the chicken and turkey even though the quantitative differences in the steroidogenic capacity between developing and mature follicles are more striking in the quail. Furthermore, although the LH-stimulated androstenedione and estrogen production appears similar in developing quail and chicken theca cells, the profile of [3H]progesterone metabolism is different in quail theca cells from that found previously in chicken theca cells.
研究了卵泡成熟对分离的日本鹌鹑颗粒细胞和膜细胞类固醇生成及类固醇代谢的影响。用促黄体生成素（LH）刺激时，最大卵泡（F1）的颗粒细胞产生的孕酮水平比未刺激时增加了六倍，而即使在最大刺激下，F3卵泡细胞的孕酮产量增加不到两倍。福斯高林刺激F1和F3颗粒细胞中的孕酮合成，但其作用不如LH明显。此外，F1细胞比F3细胞更能将25-羟基胆固醇代谢。颗粒细胞对[3H]孕酮没有明显的代谢。较小卵泡（F3-F5）的膜细胞对LH刺激的反应是产生比F1卵泡膜细胞更多的雌激素和雄烯二酮。[3H]孕酮在膜细胞中主要代谢为雄烯二酮。因此，鹌鹑颗粒细胞体外类固醇生成的总体模式与鸡和火鸡的相似，尽管发育中和成熟卵泡之间类固醇生成能力的定量差异在鹌鹑中更为显著。此外，尽管在发育中的鹌鹑和鸡的膜细胞中，LH刺激的雄烯二酮和雌激素产生似乎相似，但鹌鹑膜细胞中[3H]孕酮的代谢情况与先前在鸡膜细胞中发现的不同。
